We recommend that researchers use single copy events, and offer copy number screening at the seedling stage. It is a substantial expense to take plants to seed, so early screening provides the highest quality product in the most efficient manner. WCIC will determine the insert copy number of the events produced as they enter the greenhouse stage of the transformation process. Only single copy events will be advanced. This will ensure that subsequent expression analysis, phenotype, and segregation is not confounded by multiple copy and possibly multiple insert events, and will ensure the highest quality downstream data. Copy number analysis will be done using a Taqman platform.
Wisconsin Crop Innovation Center