Permits for Transgenic Material

APHIS Permit Application: Tips from WCIC

https://efile.aphis.usda.gov/s

*** If it is your first time submitting a permit to ship seed from WCIC, please email your application to Dayane Lima (dclima@wisc.edu) for review prior to submission.***

If planning to ship genetically modified seeds across state lines = Interstate Movement Permit

If planning to ship genetically modified seeds and plant in a field = Interstate Movement & Release Permit

Please list the WCIC as the ‘Organism Supplier or Developer’ as well as the ‘Point of Origin’

WCIC Shipping Address and Contact Information:

Selecting your Regulated Article/Organism:

Dicots:

• Glycine max (soybean)
  • Cultivar: Williams82, IL3025N, perhaps another line (need to specify)
• Vigna unguiculata (cowpea)
  • Cultivar: IT86D-1010, perhaps another line (need to specify)

Dicot Mode of Transformation: Agrobacterium rhizogenes (disarmed)

Monocots:

• Zea mays (maize)
  • Cultivar: LH244, perhaps another line (need to specify)
• Sorghum bicolor (sorghum)
  • Cultivar: Tx430, perhaps another line (need to specify)

Monocot Mode of Transformation: Agrobacterium tumefaciens (disarmed)

Means of Movement: “express carrier service with tracking (UPS, FedEx, DHL, etc.)”

Material Type and Quantity: “5 Kilograms seeds” [—- always best to OVERESTIMATE this quantity]

Constructs: You’ll also need to include a description of the elements in EACH of your constructs.

• Please include in the ‘construct name’ the workplan (WP) number assigned to your construct at the WCIC.
• Use the vector map(s) emailed to you from Ray Collier if you are unsure of the elements in your construct(s). If you need additional help, please email Emily Hahn (eameyers2@wisc.edu).

If it is required to include a design protocol for shipping, here is the language we provide:

Seeds are placed in a paper envelope that is stapled shut. Envelopes are put into a sealed corrugated cardboard box that is then placed into a sealed plastic bag consisting of either 5 mil plastic, woven polypropylene, or Mylar material. This is then placed into a sturdy corrugated cardboard shipping container. Shock absorbing cushioning material is placed into the shipping container to prevent movement during shipping.